GENOTYPING
Genotyping or DNA fingerprinting is an important tool to assist in the identification and tracking of living organisms, from bacteria and plants to animals and humans. Not just a tool for use in CSI, genotyping has many uses in agriculture, aquaculture and wildlife management from the identification of individual animals and assignment of parentage to its use as a genetic tag for animal and material traceability.
Our state-of-the-art molecular biology lab employs low to high throughput DNA extraction methods to prepare your samples for microsatellite/STR genotyping using the latest and most flexible instrumentation platforms that include:
Microsatellite or STR-based genotyping using ABI 3130XL/ABI 3500XL Genetic Analyzer
EXISTING MARKER PANELS:
Atlantic salmon continent of origin (King 7)
Atlantic salmon panels for parentage assignment and traceability
Atlantic cod
Haddock
Moose
White tailed and Mule Deer
Cannabis
Black Spruce, White Spruce and Norway Spruce
CUSTOM MARKER PANELS:
If you are in need of a marker panel for your species of interest, we can help you discover a robust and efficient marker panel to suit your needs. Alternatively, if you have a panel of your own design that you would like to run on your samples, we can also help you with that.
DOWNSTREAM ASSISTANCE:
We can help you use genotype information to develop or enhance broodstock, trace stocks within your production system, and help develop a database to store the molecular fingerprints of your genetic material for use in germline identification.
Wildlife Genotyping
For over a decade, RPC has been a trusted partner to wildlife law enforcement, providing accurate and reliable wildlife genotyping services. Our testing meets ISO 9001:2015 internationally recognized quality standards, ensuring precise results you can depend on.
SERVICES:
Species Identification
DNA based analysis to determine the species origin of a sample.
Individual Identification (Deer and Moose):
DNA based analysis to determine the unique DNA fingerprint of an individual. This analysis can be used to determine whether two samples (e.g. blood on a knife and meat in a freezer) are from the same animal.Gender Typing
DNA based analysis to determine the sex (male or female) of a sample.Expert Testimony and Consultation
RPC’s wildlife scientists and technicians may be available to provide expert testimony.
ANALYSIS CAN BE PERFORMED ON:
Meat (raw & processed)
Skin
Blood
Hair
Scat
Cannabis Genotyping
Cannabis genotyping and comparison has a variety of applications, including identifying unique cultivars or strains, verifying genetic relationships between clones and mother plants, and enabling full traceability throughout the cannabis production process.
CANNABIS GENOTYPING SERVICES:
Cannabis DNA can be extracted from a variety of different substrates such as flowers, leaf, or stems. The extracts are amplified using a novel multiplex microsatellite panel designed from markers found in published scientific literature. After amplification, the samples are electrophoresed and analyzed for allele composition. Resulting genotypes are compared, and the Random Match Probability (RMP) is used to determine the probability that two samples have arisen from the same genetic stock.
TESTING OPTIONS:
Clients can submit multiple samples for genotype comparison, or single samples for individual genotyping.
