NEXT GENERATION SEQUENCING (NGS)
Unlock the power of DNA with RPC’s next-generation sequencing services for microbiome and environmental research. We deliver fast, accurate sequencing and customized analysis to help you understand microbial communities and monitor ecosystems.
RPC’s next-generation sequencing services provide analyses for both microbiome studies and environmental DNA (eDNA) research. We employ the Illumina MiSeq sequencing platform for high-throughput sequencing delivery.
MICROBIOME ANALYSIS
Microbiome research explores how microorganisms and their genes relate to human or animal health. Through targeted 16S and ITS metabarcoding we gain insights into bacterial and fungal communities, respectively. We provide sequence data for our collaborating partners on human gut microbiome profiles, as well as participate in research on salmon gut and skin microbiomes.
ENVIRONMENTAL BIODIVERSITY OR MONITORING
We work with researchers and conservation organizations in their environmental DNA (eDNA) studies on ecosystem biodiversity. In addition to bacterial and fungal targets, we have the capacity for 12S, 18S, or COI (cytochrome c oxidase subunit I) metabarcoding for assessing fish, eukaryotic, and metazoan (animal) communities. We process a variety of environmental samples from aquatic, marine and terrestrial habitats. In environmental monitoring, bacterial community surveys can benefit the health monitoring of agriculture and aquaculture rearing sites. In addition, we can examine a variety of samples including biofilms and runoff from remedial and industrial environments.
Our collaborative team provides bioinformatics analysis tailored to your project’s needs.
16S VARIABLE REGION PRIMER SETS AVAILABLE FOR 16S METAGENOMICS:
Bacterial 16S, Illumina V3-V4 region (341F-805R)
Bacterial 16S, Earth Microbiome Project V4 region primers (515F-806R)
Bacteria and Archaea Universal V4-V5 primers (515F- 926R)
Fungal communities, ITS2
Fish communities, MiFish 12S
Metazoa/Animal communities COI (cytochrome c oxidase subunit I)
Eukaryotic 18S communities
ADDITIONAL NEXT GENERATION SEQUENCING SUPPORT SERVICES
Nucleic acid extraction:
Microbial DNA can be extracted from tissues and complex environmental samples such as soils, aquatic sediments, or water samples, as well as swabs and fecal samples.
Genomic DNA extraction for genomic projects on a wide variety of organisms.
RNA Extraction services for transcriptomic projects
Nucleic Acid quality assessment and quantitation through Nanodrop (spectrophotometric) and Qubit (fluorometric) systems
Qubit RNA Integrity assessment using the RNA IQ assay kit
